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Samford University -- Department of Biological and Environmental Sciences
Genetics -- Biol 333

Three-Point Test Cross Problems Answers

1) Gene order: BAC or CAB; Distances: B-A=3cM, A-C=20cM (so, B-C=23cM); Coefficient of coincidence=0.83, this means there was little interference: 17% interference (statistically, 5 double crossover if not significantly different from 6) (83% of expected double crossovers occurrred).
2) Gene order: ACB or BCA; Distances: A-C=10cM, C-B=14cM (so A-B=24cM); Coefficient of coincidence=0.36, this means there was a lot of interference: 64% interference (only 36% of expected double crossovers actually occurred).
3) Gene order: ABC or CBA; Distances: A-B=14cM, B-C=14cM (so A-C=28cM); Coefficient of coincidence=0.26, this means there was a lot of interference: 74% interference (only 26% of expected double crossovers actually occurred).