DNA extraction from your
fish meat sample will be using using the Wizard™ Genomic DNA
Purification Kit from Promega™:
Disinfect you work area
before you begin by wiping down with 70% ethanol (or 70%
isopropanol or bleach). Wear gloves throughout the
procedure. All glassware, plastic tubes, and utensils are
to have been sterilized. Record your protocol in your lab
notebook just as you performed it with any explanatory
notes you wish. Use a pen and be thorough. Be sure to
write the date of the procedure and the details about the
sample you are analyzing. Leave your lab notebook in the
lab (in your team's drawer). Be sure you know how to
use the pipetters before you begin. Take care not to
aspirate bubbles when drawing up solution. Discard pipette tips
after use unless pipetting the same solution into empty,
clean tubes. If there is any danger the tip is
contaminated, throw it away! Be sure the centrifuge is
balanced before starting a run. Always label your tube
with your lab team number and the date throughout the
experiment.
DNA extraction involves
1) breaking apart cellular membranes to release DNA; 2)
removal (or denaturation) of proteins (especially DNases
that would destroy DNA); 3) alcohol precipitation of DNA;
4) dissolving (rehydrating) DNA in water.
In the protocol below, we will use the Animal Tissue procedure, but we will use one adult female rather than 10-20 mg of tissue.
In the protocol below, we will use the Animal Tissue procedure, but we will use one adult female rather than 10-20 mg of tissue.