Samford University -- Department of Biological and Environmental Sciences
Cell and Molecular Biology -- Biol 405
405 Home Page Syllabus Lecture & Lab Outlines Other Stuff daj's Home Page

Molecular Techniques

  • Restriction Endonucleases: These endonucleases cut DNA at specific palindromic sites. They may be either blunt end or stagger cutters.
  • Electrophoresis: This technique separates macromolecules by size in an agarose or polyacrylamide gel.
  • Gene Cloning:
    • In vivo (E. coli)
    • In vitro (PCR)
  • Hybridization Techniques: 
    • Northern Blotting, Southern Blotting, Western Blotting: These techniques combine electrophoresis and hybridization of specific probes to identify DNA present in a sample.
    • Microarrays
 




  • DNA sequencing: 
    • Sanger sequencing
    • Next-generation sequencing