- Restriction
Endonucleases: These endonucleases cut DNA at
specific palindromic sites. They may be either blunt end
or stagger cutters.
- Electrophoresis: This
technique separates macromolecules by size in an agarose
or polyacrylamide gel.
- Gene Cloning:
- In
vivo (E. coli)
- In
vitro (PCR)
- Hybridization
Techniques:
- Northern Blotting, Southern Blotting,
Western Blotting: These techniques
combine electrophoresis and hybridization of specific
probes to identify DNA present in a sample.
- Microarrays
|
|